| A Piece of the Puzzle (10) | Unlock a piece of Subject 16's video. |
| An Old Friend Returns (100) | Escape the hideout. |
| An Unexpected Journey (30) | Complete DNA Sequence 6. |
| Arrivederci Abstergo (20) | Break out of Abstergo. |
| Art Connoisseur (10) | Buy a Painting from Florence and Venice. |
| Assassin For Hire (10) | Complete your first assassination mission for Lorenzo Il Magnifico. |
| Bianca's Man (30) | Complete DNA Sequence 10. |
| Bleeding Effect (30) | Complete training and reenter the Animus. |
| Bloody Sunday (30) | Complete DNA Sequence 4. |
| Choir Boy (20) | Discover the Assassin's Tomb inside Santa Maria del Fiore (The Duomo). |
| Doctor (20) | Perform an Air Assassination on a Poisoned NPC. |
| Exit the Son (30) | Complete DNA Sequence 3. |
| Fly Swatter (5) | Kick a Guard while using the Flying Machine. |
| Hallowed be thy name (20) | Discover the Assassin's Tomb inside the Basilica di San Marco. |
| Handy Man (10) | Upgrade a building in the Stronghold. |
| High Dive (10) | Perform a Leap of Faith from the Top of Florence's Giotto's Campanile. |
| I can see your house from here! (20) | Discover the Assassin's Tomb inside the Torre Grossa. |
| I like the view (10) | Synchronize 10 View Points. |
| In Memory of Petruccio (30) | Collect all the Feathers. |
| Kleptomaniac (10) | Pickpocket 1000 Florins. |
| Lightning Strike (10) | Sprint for 100 meters. |
| Macho Man (10) | Defend a woman's honor. |
| Mailman (10) | Intercept a Borgia Courrier. |
| Man of the People (10) | Toss more than 300 florins on the ground. |
| Masquerade (30) | Complete DNA Sequence 9. |
| Messer Sandman (10) | Stun 4 guards at once by throwing sand in their face. |
| Myth Maker (5) | Find the 8 statuettes in Monteriggioni. |
| No-hitter (20) | Kill 10 enemies while remaining in conflict without being hit. |
| Perfect Harmony (10) | Tint your clothes with those colors: Wetland Ebony and Wetland Ivory. |
| Podesta` of Monteriggioni (30) | Reach 80% of your stronghold's total value. |
| Prison Escape (20) | Discover the Assassin's Tomb inside the Rocca di Ravaldino fortress. |
| Red Light Addict (10) | Spend 5000 florins on Courtesans. |
| Show your Colors (10) | Wear the Auditore cape in each city. |
| Steal Home (10) | Win a race against thieves! |
| Street Cleaner (10) | Hide 5 dead bodies in a Bale of Hay. |
| Sweeper (10) | Sweep 5 guards at once by using a Long Weapon. |
| The Birth of an Assassin (20) | Be reborn as Ezio Auditore Da Firenze. |
| The Conspirators (30) | Complete DNA Sequence 5. |
| The Impenetrable Palazzo (30) | Complete DNA Sequence 8. |
| The Merchant of Venice (30) | Complete DNA Sequence 7. |
| The Pain of Betrayal (30) | Complete DNA Sequence 1. |
| The Prophet (30) | Complete DNA Sequence 11. |
| The Vault (30) | Complete DNA Sequence 14. |
| Tip of the Iceberg (10) | Use your Eagle Vision to scan a Glyph in the environment. |
| Undertaker (20) | Discover the Assassin's Tomb inside the catacombs under Santa Maria Novella. |
| Venetian Gladiator (20) | Discover the Assassin's Tomb inside Santa Maria della Visitazione. |
| Vengeance (30) | Complete DNA Sequence 2. |
| Victory lies in preparation (10) | Get all Hidden Blades, Item Pouches and Armor upgrades for Ezio. |
| Vitruvian Man (20) | Unlock all 20 pieces of Subject 16's video. |
| Welcome to the Animus 2.0 (20) | Enter the Animus 2.0. |
