| A Piece of the Puzzle (Bronze) | Unlock a piece of Subject 16's video. |
| An Old Friend Returns (Gold) | Escape the hideout. |
| An Unexpected Journey (Silver) | Complete DNA Sequence 6. |
| Arrivederci Abstergo (Bronze) | Break out of Abstergo. |
| Art Connoisseur (Bronze) | Buy a Painting from Florence and Venice. |
| Assassin for Hire (Bronze) | Complete your first assassination mission for Lorenzo Il Magnifico. |
| Blanca's Man (Silver) | Complete DNA Sequence 10. |
| Bleeding Effect (Silver) | Complete training and reenter the Animus. |
| Bloody Sunday (Silver) | Complete DNA Sequence 4. |
| Choir Boy (Bronze) | Discover the Assassin's Tomb inside Santa Maria del Flore (The Duomo). |
| Doctor (Bronze) | Perform an Air Assassination on a Poisoned NPC. |
| Exit the Son (Silver) | Complete DNA Sequence 3. |
| Fly Swatter (Bronze) | Kick a Guard while using the Flying Machine. |
| Hallowed be thy name (Bronze) | Discover the Assassin's Tomb inside the Basilica di San Marco. |
| Handy Man (Bronze) | Upgrade a building in the Stronghold. |
| High Dive (Bronze) | Perform a Leap of Faith from the Top of Florence's Glotto's Campanile. |
| I can see your house from here! (Bronze) | Discover the Assassin's Tomb inside Torre Grossa. |
| I like the view (Bronze) | Synchronize 10 View Points. |
| In Memory of Petruccio (Bronze) | Collect all the Feathers. |
| Kleptomaniac (Bronze) | Pickpocket 1000 Florins. |
| Lightning Strike (Bronze) | Sprint for 100 meters. |
| Macho Man (Bronze) | Defend a woman's honor. |
| Mailman (Bronze) | Intercept a Borgia Courrier. |
| Man of the People (Bronze) | Toss more than 300 florins on the ground. |
| Masquerade (Silver) | Complete DNA Sequence 9. |
| Master Assassin (Platinum) | Unlock all trophies. |
| Messer Sandman (Bronze) | Stun 4 guards at once by throwing sand in their face. |
| Myth Maker (Bronze) | Find the 8 statuettes in Monteriggioni. |
| No-hitter (Bronze) | Kill 10 enemies while remaining in conflict without being hit. |
| Perfect Harmony (Bronze) | Tint your clothes with those colors: Wetland Ebony and Wetland Ivory. |
| Podesta` of Monteriggioni (Bronze) | Reach 80% of the your stronghold's total value. |
| Prison Escape (Bronze) | Discover the Assassin's Tomb inside the Rocca di Ravaldino fortress. |
| Red Light Addict (Bronze) | Spend 5000 florins on Courtesans. |
| Show your Colors (Bronze) | Wear the Auditore cape in each city. |
| Steal Home (Bronze) | Win a race against thieves! |
| Street Cleaner (Bronze) | Hide 5 dead bodies in a Bale of Hay. |
| Sweeper (Bronze) | Sweep 5 guards at once by using a Long Weapon. |
| The Birth of an Assassin (Bronze) | Be reborn as Ezio Auditore Da Firenze. |
| The Conspirators (Silver) | Complete DNA Sequence 5. |
| The Impenetrable Palazzo (Silver) | Complete DNA Sequence 8. |
| The Merchant of Venice (Silver) | Complete DNA Sequence 7. |
| The Pain of Betrayal (Silver) | Complete DNA Sequence 1. |
| The Prophet (Silver) | Complete DNA Sequence 11. |
| The Vault (Silver) | Complete DNA Seqeunce 14. |
| Tip of the Iceberg (Bronze) | Use your Eagle Vision to scan a Glyph in the environment. |
| Undertaker (Silver) | Discover the Assassin's Tomb inside the catacombs under Santa Maria Novella. |
| Venetian Gladiator (Bronze) | Discover the Assassin's Tomb inside Santa Maria della Visitazione. |
| Vengeance (Silver) | Complete DNA Sequence 2. |
| Victory lies in preparation. (Bronze) | Get all Hidden Blades, Item Pouches and Armor upgrades for Ezio. |
| Vitruvian Man (Bronze) | Unlock all 20 pieces of Subject 16's video. |
| Welcome to the Animus 2.0 (Silver) | Enter the Animus 2.0. |
