| 'Mr. Bubbles - No!' (Bronze) | Took down your first Big Daddy in a non-private match. |
| 9-Irony (Bronze) | Paid your respects to the founder of Rapture. |
| Adopted a Little Sister (Bronze) | Adopted a new Little Sister for the very first time. |
| Against All Odds (Bronze) | Finished the game on the hardest difficulty level. |
| All Plasmids (Bronze) | Found or purchased all 11 basic Plasmid types. |
| All Weapon Upgrades (Bronze) | Found every Power to the People weapon upgrade in the game. |
| Big Brass Balls (Silver) | Finished the game without using Vita-Chambers. |
| Big Spender (Bronze) | Spent 2000 dollars at Vending Machines. |
| Bought a Slot (Bronze) | Bought one Plasmid or Tonic Slot at a Gatherer's Garden. |
| Choose the Impossible (Gold) | Achieve Rank 40. |
| Confronted Grace (Bronze) | Confronted Lamb's lieutenant in Pauper's Drop. |
| Counterattack (Bronze) | Killed an enemy with its own projectile. |
| Daddy's Home (Bronze) | Found your way back into the ruins of Rapture. |
| Dealt with Every Little Sister (Silver) | Dealt with every Little Sister. |
| Defeated the Preacher (Bronze) | Defeated the Preacher. |
| Disgusting Frankenstein (Bronze) | Become a Big Daddy for the first time in a non-private match. |
| Distance Hacker (Bronze) | Used the Hack Tool to hack an object at a distance. |
| Escape (Gold) | Escaped Rapture. |
| First Research (Bronze) | Researched a Splicer with the Research camera. |
| Found Lamb's Hideout (Bronze) | Gained access to Lamb's stronghold. |
| Fully Upgraded a Plasmid (Bronze) | Fully upgraded one Plasmid to the Level 3 version. |
| Fully Upgraded a Weapon (Bronze) | Installed the third upgrade to a weapon. |
| Grand Daddy (Silver) | Defeated 3 Big Daddies without dying during the fight. |
| Heading to the Surface (Bronze) | Headed to the surface on the side of Sinclair's escape pod. |
| Little Moth (Bronze) | Achieved Rank 20. |
| Look at You, Hacker (Bronze) | Killed 50 enemies using only hacked Security. |
| Man About Town (Bronze) | Played at least one non-private match on each multiplayer map. |
| Master Gatherer (Silver) | Gathered 600 ADAM with Little Sisters. |
| Master Hacker (Bronze) | Hacked 30 machines at a distance with the Hack Tool. |
| Master Protector (Bronze) | Got through a Gather with no damage and no one getting to the Little Sister. |
| Max Plasmid Slots (Bronze) | Fully upgraded to the maximum number of Plasmid Slots. |
| Mother Goose (Bronze) | Saved your first Little Sister in a non-private match. |
| Nose for News (Bronze) | Uncovered the secret of Dionysus Park. |
| One Research Track (Bronze) | Maxed out one Research Track. |
| Parasite (Bronze) | Achieved Rank 10. |
| Platinum Trophy | Unlocked all trophies |
| Prolific Hacker (Bronze) | Hacked one of each kind of machine. |
| Protector (Bronze) | Defended yourself against Lamb's Assault in the train station. |
| Proving Grounds (Silver) | Won your first non-private match. |
| Rapture Historian (Silver) | Found 100 audio diaries. |
| Research Master (Bronze) | Completed all research on every subject in Rapture. |
| Reunion (Silver) | Reunited with your original Little Sister. |
| Savior (Silver) | Saved every Little Sister and spared Grace, Stanley and Gil. |
| Sinclair's Solution (Bronze) | Joined forces with Sinclair in Ryan Amusements. |
| Skin Job (Bronze) | Achieved Rank 30. |
| Trap Master (Bronze) | Killed 30 enemies using only Traps. |
| Two-Bit Heroics (Bronze) | Complete your first trial in a non-private match. |
| Unbreakable (Bronze) | Defended yourself against the Big Sister without dying. |
| Unnatural Selection (Bronze) | Scored your first kill in a non-private match. |
| Upgraded a Weapon (Bronze) | Upgraded a weapon at a Power to the People Station. |
| Welcome to Rapture (Bronze) | Completed your first non-private match. |
