| Annie Radd | Complete dispatch mission "Roadie Despair" |
| Beebee | Complete the Snowy Mountains level |
| Elmira Clamp | Complete dispatch mission "Assistant Librarian" |
| Gonk | Complete dispatch mission "Gonk Need Food, Badly" |
| Gordon | Complete the Boudreaux Mansion level |
| Hopper | Complete dispatch mission "Tainted Broth" |
| King Mike | Complete the Jungle Temple level |
| Leaf | During Evelyn's house robbery case, dig around one of the holes west of the Forest Park. If you dig in the right hole, Leaf will be found |
| Liberty | Complete dispatch mission "Snake on the Loose!" |
| Lyndsay | Complete the Jungle Temple level |
| Madame Zoe | Complete the Boudreaux Mansion level |
| Magellan | Complete the Jungle Temple level |
| Makoto | Complete dispatch missions "High School Yearbook" and "Prom Date" |
| Marlon | Complete dispatch missions "Magical Assistant" and "Magical Disaster" |
| Master Aran | Complete dispatch mission "Penguin Style" |
| Ms. Nicole Vogue | Complete the Boudreaux Mansion level |
| Petal | Complete dispatch mission "Failing Forest" |
| Pinky | Complete dispatch mission "Blue Thing!" |
| Preston Winthrop | Complete the Snowy Mountains level |
| Professor Nova | Complete the Snowy Mountains level |
| Renée | Complete dispatch mission "Pig, Camera, Action!" |
| Roger | Complete the Evelyn robbery case |
| Rosalyn P. Marshall | Complete the Snowy Mountains level |
| Sir Spencer | Complete dispatch mission "H4XXOR3D!" |
| Sir Vincent Skullfinder | Complete the Jungle Temple level |
| Star | Complete dispatch mission "Blade of Destiny" |
| Travis | Available to recruit by default once you become a special agent |
| Trevor Verily | Complete the Boudreaux Mansion level |
| Vic Vector | Complete the Snowy Mountains level |
| Violet Nightshade | Complete the Boudreaux Mansion level |
| Wendalyn | Complete dispatch mission "Reagant Run" |
| Wolfah | Complete the Snowy Mountains level |
| Zombie Carl | Complete the Boudreaux Mansion level |
